University of Heidelberg and the German Cancer Research Center

1 ARTICLES PUBLISHED IN JoVE

Biology

Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography

Mila M. Leuthold^1,2, Anna D. Koromyslova^1,2, Bishal K. Singh^1,2, Grant S. Hansman^1,2

¹Schaller Research Group, University of Heidelberg and the German Cancer Research Center, ²Department of Infectious Diseases, Virology, University of Heidelberg

Here, we describe a method to express and purify high quality norovirus protruding (P) domains in E. coli for use in X-ray crystallography studies. This method can be applied to other calicivirus P domains, as well as non-structural proteins, i.e., viral protein genome-linked (VPg), protease, and RNA dependent RNA polymerase (RdRp).